User:Matt Hartings/Notebook/Photosynthesis/2013/01/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | |style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==MOF== | ||
Abigail and I are trying a new MOF synthesis. | |||
51.8 mg DO2A | |||
61.0 mg Zn(NO3)2 6H20 | |||
3 mL of diethylformamide | |||
in a scint vial with a teflon cap | |||
sonicate to create a suspension | |||
Place in oven at 130C at 9:45am | |||
Abigail and I checked the reaction at 12pm, the solution was brown. This is much different than the first time we performed the reaction, where there was a clear solution with a percipitate. We are going to let it run until 1 and then let it cool. | |||
==Transformation== | |||
# Make plates | |||
## 2.5g LB | |||
## 2.0g Agar | |||
## 100mL water | |||
## autoclave | |||
## add 100uL of 100mg/ml ampicilin | |||
## Pour plates | |||
# | |||
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Latest revision as of 22:24, 26 September 2017
 Protein Re-engineering
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MOFAbigail and I are trying a new MOF synthesis. 51.8 mg DO2A 61.0 mg Zn(NO3)2 6H20 3 mL of diethylformamide in a scint vial with a teflon cap sonicate to create a suspension Place in oven at 130C at 9:45am Abigail and I checked the reaction at 12pm, the solution was brown. This is much different than the first time we performed the reaction, where there was a clear solution with a percipitate. We are going to let it run until 1 and then let it cool. Transformation
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