User:Matt Hartings/Notebook/Photosynthesis/2012/09/13: Difference between revisions
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==Objective== | ==Objective== | ||
Extract Hb protein from cells. | |||
==Procedure== | |||
#Thaw cell pellets | |||
#Run 3 cycles of ultrasonication | |||
##30seconds sonicating | |||
##30 seconds on ice | |||
#Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water | |||
#centrifuge at 18000rpm at 4C for 2 hours | |||
#decant liquid from tubes into fresh falcon tubes. | |||
#store in refrigerator | |||
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Revision as of 10:04, 17 September 2012
 Protein Re-engineering
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ObjectiveExtract Hb protein from cells. Procedure
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