Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2012/09/11"

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(Objective)
(Objective)
Line 11: Line 11:
  
 
==Objective==
 
==Objective==
Stuff
+
Prep for hemoglobin expression tomorrow
 +
 
 +
==Procedure==
 +
Prepare autoclaved LB broth
 +
#Place 25 g of LB in Fernbach flask
 +
#Add 1 L of water
 +
#Repeat to make a total of 4 flasks
 +
#Place 0.875g of LB in 250mL ehrlenmeyer flask
 +
#Add 35mL of water
 +
#Repeat to make a total of 4 flasks
 +
#Autoclave flasks
 +
##2 runs each containing 2 fernbachs and 2ehrlenmeyers
 +
 
 +
Inoculate starter cultures
 +
#Add 35uL of 100mg/mL ampicilin to each ehrlenmeyer
 +
#Transfer culture from frozen glycerol stock of WT Ascaris Hb to starter culture flasks
 +
#Shake overnight at 37C
  
  

Revision as of 07:22, 12 September 2012

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Objective

Prep for hemoglobin expression tomorrow

Procedure

Prepare autoclaved LB broth

  1. Place 25 g of LB in Fernbach flask
  2. Add 1 L of water
  3. Repeat to make a total of 4 flasks
  4. Place 0.875g of LB in 250mL ehrlenmeyer flask
  5. Add 35mL of water
  6. Repeat to make a total of 4 flasks
  7. Autoclave flasks
    1. 2 runs each containing 2 fernbachs and 2ehrlenmeyers

Inoculate starter cultures

  1. Add 35uL of 100mg/mL ampicilin to each ehrlenmeyer
  2. Transfer culture from frozen glycerol stock of WT Ascaris Hb to starter culture flasks
  3. Shake overnight at 37C