Difference between revisions of "User:Matt Hartings/Notebook/Photosynthesis/2012/06/28"

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(Objective)
(Objective)
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==Objective==
 
==Objective==
Prepare media for hemoglobin expression.
+
Prepare media for hemoglobin expression and set up starter cultures.
  
 +
==Procedure==
 
Starter cultures (x4)
 
Starter cultures (x4)
 
# 0.875g LB
 
# 0.875g LB
Line 22: Line 23:
 
# 1L water
 
# 1L water
 
# autoclave
 
# autoclave
 +
 +
Starter cultures (x4)
 +
# 35uL of 100mg/mL ampicilin was added to each starter culture flask
 +
# Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock
 +
# Flasks were placed in the shaker/incubator overnight (37C, 250rpm)
  
  

Revision as of 12:18, 30 June 2012

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Objective

Prepare media for hemoglobin expression and set up starter cultures.

Procedure

Starter cultures (x4)

  1. 0.875g LB
  2. 35mL water
  3. autoclave

Expression cultures (x4)

  1. 25g LB
  2. 1L water
  3. autoclave

Starter cultures (x4)

  1. 35uL of 100mg/mL ampicilin was added to each starter culture flask
  2. Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock
  3. Flasks were placed in the shaker/incubator overnight (37C, 250rpm)