Difference between revisions of "User:Matt Hartings/Notebook/Phosphorylation/2012/09/17"

From OpenWetWare
Jump to: navigation, search
(Objective)
(Description)
Line 14: Line 14:
  
 
==Description==
 
==Description==
# Add experimental record here. Include what, how, and why...
+
Following protocol [http://cfs.umbi.umd.edu/cfs/reprints/Long-Lifetime%20Ru(II)%20Complexes%20as%20Labeling.pdf Lackowicz paper]
 +
#1mmol of [Ru(Cl)<sub>2</sub>(bpy)<sub>2</sub>] added to 50mL roundbottom (0.4816g)
 +
#1mmol of 1,10-phenanthroline 5-amine added to 50mL roundbottom (0.1974g)
 +
#25mL of methanol added
 +
#Reaction run under N<sub>2</sub> and refluxed for 3 hours (started at 11 am)
  
 
==Data==
 
==Data==

Revision as of 09:14, 17 September 2012

Hartings AU Phosphorylation Header.png Phosphorylation Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>



Objective

Synthesize new Ru(bpy)2(phen-NH2) for ATP synthesis.

Description

Following protocol Lackowicz paper

  1. 1mmol of [Ru(Cl)2(bpy)2] added to 50mL roundbottom (0.4816g)
  2. 1mmol of 1,10-phenanthroline 5-amine added to 50mL roundbottom (0.1974g)
  3. 25mL of methanol added
  4. Reaction run under N2 and refluxed for 3 hours (started at 11 am)

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.