Difference between revisions of "User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/22"

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# Gold
 
# Gold
 
## Need 50mL of 2.5mM
 
## Need 50mL of 2.5mM
 +
## 49.7mg of HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water --> 2.52 mM
  
 
==Notes==
 
==Notes==

Revision as of 08:41, 22 October 2013

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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Description

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Data

Stock Solutions

  1. HRP
    1. 67.3mg in 100mL of water ---> 15.3 μM
  2. Pepsin
    1. 54.4mg in 100mL of water ---> 15.7 μM
  3. Gold
    1. Need 50mL of 2.5mM
    2. 49.7mg of HAuCl4·3H2O in 50mL water --> 2.52 mM

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.