Difference between revisions of "User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/15"

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(Data)
(Data)
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Stock solutions
 
Stock solutions
 
# Gold
 
# Gold
## 25.0mg HAuCl<sub>4</sub>·H<sub>2</sub>O in 25.0mL water --> 29.4mM
+
## 25.0mg HAuCl<sub>4</sub>·H<sub>2</sub>O in 25.0mL water --> 2.94mM
 
# HRP
 
# HRP
 
## 17.9mg HRP in 25.0mL water --> 16.27μM
 
## 17.9mg HRP in 25.0mL water --> 16.27μM

Revision as of 08:21, 15 October 2013

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Objective

Today we are going to be making HRP-AuNPs for enzymatic studies.

Description

  1. Make 4 solutions of HRP-AuNPs
    1. Au:HRP ratio - 30:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    2. Au:HRP ratio - 60:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    3. Au:HRP ratio - 90:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)
    4. Au:HRP ratio - 120:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL)

Data

Stock solutions

  1. Gold
    1. 25.0mg HAuCl4·H2O in 25.0mL water --> 2.94mM
  2. HRP
    1. 17.9mg HRP in 25.0mL water --> 16.27μM

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.