Difference between revisions of "User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/17"

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(Data)
(Description)
Line 16: Line 16:
 
I made two stock solutions  
 
I made two stock solutions  
  
Au: 20.3mg of HAuCl<sub>4</sub>·H<sub>2</sub>O in 24mL of water and
+
Au: 20.3mg of HAuCl<sub>4</sub>·H<sub>2</sub>O in 24mL of water (2.36mM) and
  
BSA: 47.2mg of BSA in 45mL of water.
+
BSA: 47.2mg of BSA in 45mL of water (15.7uM).
  
 
Samples were made in the following manner using similar ratios to the experiments I ran on [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2012/05/23|May 23rd]]
 
Samples were made in the following manner using similar ratios to the experiments I ran on [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2012/05/23|May 23rd]]
Line 34: Line 34:
 
|8.334
 
|8.334
 
|0.666
 
|0.666
|?
+
|18.0
 
|-
 
|-
 
|<b>30</b>
 
|<b>30</b>
Line 40: Line 40:
 
|5.554
 
|5.554
 
|3.446
 
|3.446
|?
+
|27.1
 
|-
 
|-
 
|<b>40</b>
 
|<b>40</b>
Line 46: Line 46:
 
|4.166
 
|4.166
 
|4.834
 
|4.834
|?
+
|36.1
 
|-
 
|-
 
|<b>50</b>
 
|<b>50</b>
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|3.334
 
|3.334
 
|5.666
 
|5.666
|?
+
|45.1
 
|-
 
|-
 
|<b>60</b>
 
|<b>60</b>
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|2.778
 
|2.778
 
|6.222
 
|6.222
|?
+
|54.1
 
|-
 
|-
 
|<b>70</b>
 
|<b>70</b>
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|2.38
 
|2.38
 
|6.62
 
|6.62
|?
+
|63.1
 
|-
 
|-
 
|<b>80</b>
 
|<b>80</b>
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|2.084
 
|2.084
 
|6.916
 
|6.916
|?
+
|72.1
 
|-
 
|-
 
|<b>90</b>
 
|<b>90</b>
Line 76: Line 76:
 
|1.854
 
|1.854
 
|7.146
 
|7.146
|?
+
|81.1
 
|-
 
|-
 
|<b>100</b>
 
|<b>100</b>
Line 82: Line 82:
 
|1.666
 
|1.666
 
|7.334
 
|7.334
|?
+
|90.2
 
|-
 
|-
 
|<b>110</b>
 
|<b>110</b>
Line 88: Line 88:
 
|1.514
 
|1.514
 
|7.486
 
|7.486
|?
+
|99.3
 
|-
 
|-
 
|<b>120</b>
 
|<b>120</b>
Line 94: Line 94:
 
|1.386
 
|1.386
 
|7.614
 
|7.614
|?
+
|108.5
 
|-
 
|-
 
|<b>130</b>
 
|<b>130</b>
Line 100: Line 100:
 
|1.28
 
|1.28
 
|7.72
 
|7.72
|?
+
|117.4
 
|-
 
|-
 
|<b>140</b>
 
|<b>140</b>
Line 106: Line 106:
 
|1.188
 
|1.188
 
|7.812
 
|7.812
|?
+
|126.5
 
|-
 
|-
 
|<b>150</b>
 
|<b>150</b>
Line 112: Line 112:
 
|1.112
 
|1.112
 
|7.888
 
|7.888
|?
+
|135.2
 
|-
 
|-
 
|<b>160</b>
 
|<b>160</b>
Line 118: Line 118:
 
|1.04
 
|1.04
 
|7.96
 
|7.96
|?
+
|144.5
 
|-
 
|-
 
|<b>170</b>
 
|<b>170</b>
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|0.98
 
|0.98
 
|8.02
 
|8.02
|?
+
|153.4
 
|-
 
|-
 
}
 
}

Revision as of 08:40, 17 September 2012

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Objective

Prep AuNP samples for AA analysis tomorrow.

Description

I made two stock solutions

Au: 20.3mg of HAuCl4·H2O in 24mL of water (2.36mM) and

BSA: 47.2mg of BSA in 45mL of water (15.7uM).

Samples were made in the following manner using similar ratios to the experiments I ran on May 23rd

I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs.

}

Data

Notes



Approx. Au/BSA ratio Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) Actual Ratio
20 1.00 8.334 0.666 18.0
30 1.00 5.554 3.446 27.1
40 1.00 4.166 4.834 36.1
50 1.00 3.334 5.666 45.1
60 1.000 2.778 6.222 54.1
70 1.00 2.38 6.62 63.1
80 1.00 2.084 6.916 72.1
90 1.00 1.854 7.146 81.1
100 1.00 1.666 7.334 90.2
110 1.00 1.514 7.486 99.3
120 1.00 1.386 7.614 108.5
130 1.00 1.28 7.72 117.4
140 1.00 1.188 7.812 126.5
150 1.00 1.112 7.888 135.2
160 1.000 1.04 7.96 144.5
170 1.00 0.98 8.02 153.4