User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/13: Difference between revisions
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==Description== | ==Description== | ||
# | #Place culture tubes on ice (6 tubes total for use with different volume of cells and volume of DNA) | ||
##30uL cells (direct from supplier), 10uL of DNA (from Hartings workup) | |||
##30uL cells (direct from supplier), 10uL of DNA (from Costanzi workup) | |||
##30uL cells (made by Tamra), 10uL of DNA (from Hartings workup) | |||
##30uL cells (made by Tamra), 10uL of DNA (from Costanzi workup) | |||
##30uL cells (made by Tamra), 5uL of DNA (from Hartings workup) | |||
##30uL cells (made by Tamra), 5uL of DNA (from Costanzi workup) | |||
#Thaw cells | |||
#Add appropriate amount of DNA | |||
#Add appropriate cells | |||
#Incubate on ice for 30minutes | |||
#Heat shock at 42C for 30seconds | |||
#Place on ice | |||
#Add 250uL of SOC | |||
#Shake at 37C for 1 hour | |||
#Plate cells. | |||
==Data== | ==Data== |
Revision as of 08:56, 17 September 2012
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