Difference between revisions of "User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/13"

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(Objective)
(Description)
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==Description==
 
==Description==
# Add experimental record here. Include what, how, and why...
+
#Place culture tubes on ice (6 tubes total for use with different volume of cells and volume of DNA)
 +
##30uL cells (direct from supplier), 10uL of DNA (from Hartings workup)
 +
##30uL cells (direct from supplier), 10uL of DNA (from Costanzi workup)
 +
##30uL cells (made by Tamra), 10uL of DNA (from Hartings workup)
 +
##30uL cells (made by Tamra), 10uL of DNA (from Costanzi workup)
 +
##30uL cells (made by Tamra), 5uL of DNA (from Hartings workup)
 +
##30uL cells (made by Tamra), 5uL of DNA (from Costanzi workup)
 +
#Thaw cells
 +
#Add appropriate amount of DNA
 +
#Add appropriate cells
 +
#Incubate on ice for 30minutes
 +
#Heat shock at 42C for 30seconds
 +
#Place on ice
 +
#Add 250uL of SOC
 +
#Shake at 37C for 1 hour
 +
#Plate cells.
  
 
==Data==
 
==Data==

Revision as of 07:56, 17 September 2012

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Objective

Transformation from yesterday didn't work. Try again using protocol from invitrogen.

Description

  1. Place culture tubes on ice (6 tubes total for use with different volume of cells and volume of DNA)
    1. 30uL cells (direct from supplier), 10uL of DNA (from Hartings workup)
    2. 30uL cells (direct from supplier), 10uL of DNA (from Costanzi workup)
    3. 30uL cells (made by Tamra), 10uL of DNA (from Hartings workup)
    4. 30uL cells (made by Tamra), 10uL of DNA (from Costanzi workup)
    5. 30uL cells (made by Tamra), 5uL of DNA (from Hartings workup)
    6. 30uL cells (made by Tamra), 5uL of DNA (from Costanzi workup)
  2. Thaw cells
  3. Add appropriate amount of DNA
  4. Add appropriate cells
  5. Incubate on ice for 30minutes
  6. Heat shock at 42C for 30seconds
  7. Place on ice
  8. Add 250uL of SOC
  9. Shake at 37C for 1 hour
  10. Plate cells.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.