Difference between revisions of "User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/12"

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(Objective)
(Description)
Line 14: Line 14:
  
 
==Description==
 
==Description==
# Add experimental record here. Include what, how, and why...
+
Following transformation protocol I normally use:
 +
#Place plastic culture tubes on ice for 15 minutes
 +
#Add cells to ice to thaw
 +
#Add 1uL of DNA to tubes
 +
#Place on ice for 1 minute
 +
#Place 30uL of cells over DNA
 +
#Incubate for 4 minutes
 +
#Heat shock for 80 seconds
 +
#Put cells on ice
 +
#Add 100uL of SOC
 +
#Shake at 37C for 1 hr
 +
#Plate on kanamycin LB plates
  
 
==Data==
 
==Data==

Revision as of 07:52, 17 September 2012

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Objective

Transform BL21(DE3) cells with the DNA extracted yesterday.

Description

Following transformation protocol I normally use:

  1. Place plastic culture tubes on ice for 15 minutes
  2. Add cells to ice to thaw
  3. Add 1uL of DNA to tubes
  4. Place on ice for 1 minute
  5. Place 30uL of cells over DNA
  6. Incubate for 4 minutes
  7. Heat shock for 80 seconds
  8. Put cells on ice
  9. Add 100uL of SOC
  10. Shake at 37C for 1 hr
  11. Plate on kanamycin LB plates

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.