User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14

From OpenWetWare
< User:Mary Mendoza‎ | Notebook‎ | CHEM 571 Experimental Biological Chemistry I‎ | 2012‎ | 11
Revision as of 11:28, 9 December 2012 by Mary Mendoza (talk | contribs) (ADA Kinetic Assay Runs)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

ADA Kinetic Assay Runs

  • During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
  • It was concluded from the previous period that the absorption of adenosine should decrease over time. A kinetic assay scan of the reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.
  • The program was set to Kinetics with the wavelength at 235 for a collection span of 600s.


Adeno300.png

  • In the scan, it can be observed that the absorption of adenosine is not decreasing over time. Instead, the absorption is increasing. Also, the signal for the absorption of adenosine is over 1.
  • It was decided that the next measurements, the concentration of adenosine should be decreased by decreasing the volume in th cuvetter. This is to ensure that the absorbance is below 1.