User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14: Difference between revisions
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* Running the UV-vis scan for each reagent showed that the increasing trend observed on the ADA kinetic assays was inosine not adenosine. This was verified by recalculating the molar absorptivities. The molar absortivity of inosine at 235 is greater than adenosine. This explains the increase over time since as ADA catalyzes the formation of inosine from adenosine; the catalysis increases the concentration of inosine so as its absorbance. | * Running the UV-vis scan for each reagent showed that the increasing trend observed on the ADA kinetic assays was inosine not adenosine. This was verified by recalculating the molar absorptivities. The molar absortivity of inosine at 235 is greater than adenosine. This explains the increase over time since as ADA catalyzes the formation of inosine from adenosine; the catalysis increases the concentration of inosine so as its absorbance. | ||
* The kinetic assay with its corrected information will be continued a week after break. | |||
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Revision as of 13:14, 9 December 2012
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ADA Kinetic Assay Runs
UV-vis of Adenosine and Inosine
For adenosine
For inosine
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