Difference between revisions of "User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14"

From OpenWetWare
Jump to: navigation, search
(ADA Kinetic Assay Runs)
(ADA Kinetic Assay Runs)
Line 8: Line 8:
 
==ADA Kinetic Assay Runs==
 
==ADA Kinetic Assay Runs==
 
* During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
 
* During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
* It was concluded from the previous period that the absorption of adenosine should decrease over time.
+
* It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.
 +
 
 +
[[Image:Adeno300.png]]
  
  

Revision as of 10:21, 9 December 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

ADA Kinetic Assay Runs

  • During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
  • It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.

Adeno300.png