User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14

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ADA Kinetic Assay Runs

  • During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
  • It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.


Adeno300.png

  • In the UV-vis scan, it can be observed that the absorption of adenosine is not decreasing over time. Instead, the absorption is increasing. Also, the signal for the absorption of adenosine is over 1.
  • It was decided that the next measurem