User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24: Difference between revisions
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* After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | * After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | ||
* Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | * Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | ||
* The tubes containing the media were | * The tubes containing the media were placed on a shaker for 1 h. at 37°C by Dr. Miller. | ||
* During the latter of the lab period, 2 plates were inoculated for each cell grown in LB and SOC media. | * During the latter of the lab period, 2 plates were inoculated for each cell grown in LB and SOC media. | ||
* The first plate was inoculated with 50 μL of the BL21 cell grown in LB; the second plate was inoculate with a greater volume of cells with 200 μL. | * The first plate was inoculated with 50 μL of the BL21 cell grown in LB; the second plate was inoculate with a greater volume of cells with 200 μL. | ||
* Dhea Patel plated the BL21 cells grown in SOC media. | * Dhea Patel plated the BL21 cells grown in SOC media. | ||
==UV-Vis of Au/lysozyme solution== | ==UV-Vis of Au/lysozyme solution== | ||
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* The trend seen on the graph is accurate with the observations made from the physical state of the Au/BSA solutions. Mole ratio 60, 80, and 120 were the solutions that exhibited no fibers and had the consistent clear purple color. | * The trend seen on the graph is accurate with the observations made from the physical state of the Au/BSA solutions. Mole ratio 60, 80, and 120 were the solutions that exhibited no fibers and had the consistent clear purple color. | ||
* On the graph, mole ratios 60, 80, and 120 have high absorbances. According to Beer's law, absorbance is directly proportional to the molar absorptivity, path length, and concentration of the substance. Therefore, mole ratio 120 displaying the greatest absorbance peak has the greatest concentration of aqueous gold nanoparticles in solution. This is followed by mole ratio 80 and 60. | * On the graph, mole ratios 60, 80, and 120 have high absorbances. According to Beer's law, absorbance is directly proportional to the molar absorptivity, path length, and concentration of the substance. Therefore, mole ratio 120 displaying the greatest absorbance peak has the greatest concentration of aqueous gold nanoparticles in solution. This is followed by mole ratio 80 and 60. | ||
* Mole ratios 130 and 133 had fibers suspended in a clear and colorless solution. These solutions can be seen having some absorbance at the 510 nm region. | |||
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Revision as of 13:39, 30 November 2012
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Transformation of BL21 cellsThe following procedures were conducted near the proximity of the Bunsen burner in accordance with aseptic techniques. The procedure is based from the laboratory Transformation protocol.
UV-Vis of Au/lysozyme solution
UV-Vis scans of Au/BSA
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