User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* The plasmid with the loading dye were inserted to the wells 3 and 4 using an automatic delivery pipet. | * The plasmid with the loading dye were inserted to the wells 3 and 4 using an automatic delivery pipet. | ||
* The Horton 5B Electrophoresis system (chamber containing the tray) was connected to the BioRad power supply powerpac 200 at 85V. | * The Horton 5B Electrophoresis system (chamber containing the tray) was connected to the BioRad power supply powerpac 200 at 85V. | ||
* Completion of the electrophoresis was followed by the addition of ethidium bromide (EtBr). Great caution was done in handling EtBr since this agent is carcinogenic by binding to any DNA in contact. | |||
* 20 μL of the 12.3 g/mL of EtBR was added to the gel suspended in TAE placed on a microwaveable plastic container. This was stirred in a VWR analog shaker at a slow speed. | |||
==UV-Vis for Chemiluminescence== | ==UV-Vis for Chemiluminescence== |
Revision as of 10:31, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
Preparation of Lysozyme stock
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