Difference between revisions of "User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/10"

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(Background of Adenosine Deaminase)
(Primer Assignment)
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| colspan="2"|
 
| colspan="2"|
 
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==Adenosine Deaminase==
 +
* Background and configuration of adenosine deaminase (ADA) were discussed by Dr. Costanzi.
 +
* A molecular visualization software, Maestro, was introduced.
 +
 
==Primer Assignment==
 
==Primer Assignment==
  
 
{| {{table}}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|''properties''
+
| align="center" style="background:#f0f0f0;"|''ADA E34A reverse primer''
| align="center" style="background:#f0f0f0;"|''4-iodophenol''
+
|-
| align="center" style="background:#f0f0f0;"|''AAP''
+
| 5' GTT AAA TCT AAA TGG CAA TGT AAT GCA 3'
| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O<sub>2</sub>''
+
|-
| align="center" style="background:#f0f0f0;"|''HRP''
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| Tm = 62.9°C MW= 15, 067.8 g/mole
 
|-
 
|-
| Molarity||18 mM||156.25 μM||1.7 mM||2.3 μM
+
| 14.6 OD = 260 nm
 
|-
 
|-
| Volume||10 μL|| ||750 μL||50 μL
+
| 30.4 nm = 0.46 mg
 
|-
 
|-
 
  
 
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Revision as of 10:50, 17 October 2012

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Adenosine Deaminase

  • Background and configuration of adenosine deaminase (ADA) were discussed by Dr. Costanzi.
  • A molecular visualization software, Maestro, was introduced.

Primer Assignment

ADA E34A reverse primer
5' GTT AAA TCT AAA TGG CAA TGT AAT GCA 3'
Tm = 62.9°C MW= 15, 067.8 g/mole
14.6 OD = 260 nm
30.4 nm = 0.46 mg