User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/03: Difference between revisions
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* Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate. | |||
* Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate as shown below. | |||
* Au/Citrate was made from 20 mL of 1 mM HAuCl<sub>4</sub> with 20 mL of 1% citrate. The Au/Citrate was added in multiple of 20. Each 20 μL is equivalent to 2%. | |||
[[Image:Aucit.png]] | [[Image:Aucit.png]] | ||
* The amount and concentration of reactants are shown below. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Trials''' | |||
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|- | |||
| Cuvet||hydrogen peroxide concentration (mM)||Iodophenol in DMSO concentration (μM)||AAP Concentration (μM)||HRP concentration (μM)||μL of gold np added||μL of water added | |||
|- | |||
| 1( standard)||1.7||18||156.25||3.45||0||630 | |||
|- | |||
| 1||1.7||18||156.25||3.45||20||610 | |||
|- | |||
| 2||1.7||18||156.25||3.45||40||590 | |||
|- | |||
| 3||1.7||18||156.25||3.45||60||570 | |||
|- | |||
| 4||1.7||18||156.25||3.45||80||550 | |||
|- | |||
| 5||1.7||18||156.25||3.45||100||530 | |||
|- | |||
| Total volume added||750 μL||10 μL||35 μL||33 μL|||| | |||
|} | |||
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Revision as of 13:49, 11 December 2012
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Kinetics of AuNP (Citrate)
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