User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 8: Line 8:
==Kinetics of AuNP (Citrate)==
==Kinetics of AuNP (Citrate)==
* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
* As suggested by Dr. Miller, the group addressed the issue by observing the problem in terms of molarity instead of volume.  
* Trial and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.
* By comparing the molarity of the reactants,   
 
[[Image:HRP.png]] 


*'''[[User:Abigail E. Miller|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.




Revision as of 13:38, 11 December 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Kinetics of AuNP (Citrate)

  • As mentioned in the previous entry of Oct. 2, there was difficulty in finding the appropriate ratio between AAP and HRP.
  • Trial and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/03&oldid=663305"