User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/03

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Revision as of 12:45, 11 December 2012 by Mary Mendoza (talk | contribs) (Kinetics of AuNP (Citrate))
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Kinetics of AuNP (Citrate)

  • As mentioned in the previous entry of Oct. 2, there was difficulty in finding the appropriate ratio between AAP and HRP.
  • Using the Kinetics program, trial and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.

HRP.png


  • Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate.


Aucit.png


  • The amount and concentration of reactants are shown below.
Trials ' ' ' ' ' '
Cuvet hydrogen peroxide concentration (mM) Iodophenol in DMSO concentration (μM) AAP Concentration (μM) HRP concentration (μM) μL of gold np added μL of water added
1( standard) 1.7 18 156.25 3.45 0 630
1 1.7 18 156.25 3.45 20 610
2 1.7 18 156.25 3.45 40 590
3 1.7 18 156.25 3.45 60 570
4 1.7 18 156.25 3.45 80 550
5 1.7 18 156.25 3.45 100 530
Total volume added 750 μL 10 μL 35 μL 33 μL