User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02

From OpenWetWare
< User:Mary Mendoza‎ | Notebook‎ | CHEM 571 Experimental Biological Chemistry I‎ | 2012‎ | 10
Revision as of 17:58, 6 October 2012 by Mary Mendoza (talk | contribs) (Preparation of the Reactants of HRP Oxidase Assay)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Preparation of the Reactants of HRP Oxidase Assay

  • The reactants listed in HRP Oxidase Assay were weighed out.
  • The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group.
  • Calculations are listed below:

0.020 mol of Na2HPO4 × Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{268.07g}{1mol}} of Na2HPO4 = 53.61 g of Na2HPO4