Difference between revisions of "User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02"

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(Preparation of the Reactants of HRP Oxidase Assay)
(Preparation of the Reactants of HRP Oxidase Assay)
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x<sub>60 mL</sub> = <math>\frac{.060 L * 53. 61 g}{1L}</math>
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x<sub>60 mL</sub> = <math>\frac{.060 L * 53. 61 g}{1L}</math> = 3.22 g of Na<sub>2</sub>HPO<sub>4</sub>
  
 
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Revision as of 18:34, 6 October 2012

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Preparation of the Reactants of HRP Oxidase Assay

  • The reactants listed in HRP Oxidase Assay were weighed out.
  • The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
  • Calculations are listed below:

0.020 mol of Na2HPO4 × Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{268.07g}{1mol}} of Na2HPO4 = 53.61 g of Na2HPO4


x60 mL = Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{.060 L * 53. 61 g}{1L}} = 3.22 g of Na2HPO4