Difference between revisions of "User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02"

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(Preparation of the Reactants of HRP Oxidase Assay)
(Preparation of the Reactants of HRP Oxidase Assay)
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==Preparation of the Reactants of HRP Oxidase Assay==
 
==Preparation of the Reactants of HRP Oxidase Assay==
 
* The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out.  
 
* The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out.  
* The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group.  
+
* The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The volume chosen for the buffer 
 
* Calculations are listed below:
 
* Calculations are listed below:
  

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Preparation of the Reactants of HRP Oxidase Assay

  • The reactants listed in HRP Oxidase Assay were weighed out.
  • The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The volume chosen for the buffer
  • Calculations are listed below:

0.020 mol of Na2HPO4 × [math]\frac{268.07g}{1mol}[/math] of Na2HPO4 = 53.61 g of Na2HPO4