Difference between revisions of "User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02"

From OpenWetWare
Jump to: navigation, search
(Preparation of the Reactants of HRP Oxidase Assay)
(Preparation of the Reactants of HRP Oxidase Assay)
(One intermediate revision by the same user not shown)
Line 9: Line 9:
 
* The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out.  
 
* The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out.  
 
* The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.   
 
* The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.   
* Calculations are listed below:
+
* Calculation for obtaining Na<sub>2</sub>HPO<sub>4</sub>:
  
 
0.020 mol of Na<sub>2</sub>HPO<sub>4</sub> × <math>\frac{268.07g}{1mol}</math> of Na<sub>2</sub>HPO<sub>4</sub> = 53.61 g of Na<sub>2</sub>HPO<sub>4</sub>
 
0.020 mol of Na<sub>2</sub>HPO<sub>4</sub> × <math>\frac{268.07g}{1mol}</math> of Na<sub>2</sub>HPO<sub>4</sub> = 53.61 g of Na<sub>2</sub>HPO<sub>4</sub>
Line 15: Line 15:
  
 
x<sub>60 mL</sub> = <math>\frac{.060 L * 53. 61 g}{1L}</math> = 3.22 g of Na<sub>2</sub>HPO<sub>4</sub>
 
x<sub>60 mL</sub> = <math>\frac{.060 L * 53. 61 g}{1L}</math> = 3.22 g of Na<sub>2</sub>HPO<sub>4</sub>
 +
 +
 +
* The required weight for 4-aminoantipyrine is 0.810 g
 +
 +
 +
 +
 +
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 19:14, 6 October 2012

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Preparation of the Reactants of HRP Oxidase Assay

  • The reactants listed in HRP Oxidase Assay were weighed out.
  • The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
  • Calculation for obtaining Na2HPO4:

0.020 mol of Na2HPO4 × Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{268.07g}{1mol}} of Na2HPO4 = 53.61 g of Na2HPO4


x60 mL = Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{.060 L * 53. 61 g}{1L}} = 3.22 g of Na2HPO4


  • The required weight for 4-aminoantipyrine is 0.810 g