User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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[[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | [[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | ||
* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the flask was connected to a Welch vacuum pump. | * The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the flask was connected to a Welch vacuum pump. | ||
* | * The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | ||
* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen | * The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random. | ||
Revision as of 02:19, 5 October 2012
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Lysis of transformed E. coli
Filtration of Buffers
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