User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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* Upon finishing sonication of cells, the samples were transferred to centrifuge tubes. The rotor chosen for the tubes is the make of Fiberlite (Piramoon Technologies Inc.) rotor F21-8x50y max. 21,000 rpm. | * Upon finishing sonication of cells, the samples were transferred to centrifuge tubes. The rotor chosen for the tubes is the make of Fiberlite (Piramoon Technologies Inc.) rotor F21-8x50y max. 21,000 rpm. | ||
* The samples contained in the centrifuge tubes were balanced on the rotor having one of the tubes contain water (total of four tubes). The centrifuge settings were fixed on 18,000 rpm at 4°C for 2 h. | * The samples contained in the centrifuge tubes were balanced on the rotor having one of the tubes contain water (total of four tubes). The centrifuge settings were fixed on 18,000 rpm at 4°C for 2 h. | ||
* The centrifuge is necessary to separate the water soluble from the water insoluble contents. The supernatant is the desired phase from this centrifuge because this contains the water soluble proteins expressed by ' E. coli'. | * The centrifuge is necessary to separate the water soluble from the water insoluble contents. The supernatant is the desired phase from this centrifuge because this contains the water soluble proteins (ADA) expressed by ''E. coli''. | ||
==Filtration of Buffers== | ==Filtration of Buffers== |
Revision as of 23:49, 4 October 2012
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Lysis of transformed E. coli
Filtration of Buffers |