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| |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} |
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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Objective==
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| * To obtain a spectrum of 40 μM adenosine and inosine
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| * A run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
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| * Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
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| * Produce histogram
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| ==Procedure for Adenosine and Inosine Spectrum==
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| * To obtain spectrum of 40 μM adenosine, dilute 600 μL of 200 μM adenosine stock solution in 2400 μL 0.05 M phosphate buffer.
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| * Stock solution of 200 μM inosine (MW 268.2 g/mol) was prepared. A spectrum was taken using the same dilution mentioned above.
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| ==Protocol==
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| * The reagents were added in the following sequence:
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| ** 1.78 mL of phosphate buffer
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| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
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| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
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| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
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| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
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| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
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| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
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| * The kinetics of the reaction was taken using the UVProbe software.
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| *Cuvette Mixture no Inhibitor
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| [[Image:CuvetteMixtureNoInhibitor.png]]
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| *Cuvette Mixture with Inhibitor
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| [[Image:CuvetteSolnInhibitorHisto.png]]
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| *Inhibitors Ran today:
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| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Project name
Main project page
Previous entry
