Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/17"

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(Objective)
(Protocol)
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==Protocol==
 
* The reagents were added in the following sequence:
 
** 1.78 mL of phosphate buffer
 
** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
* The kinetics of the reaction was taken using the UVProbe software.
 
  
*Cuvette Mixture no Inhibitor
 
[[Image:CuvetteMixtureNoInhibitor.png]]
 
 
*Cuvette Mixture with Inhibitor
 
[[Image:CuvetteSolnInhibitorHisto.png]]
 
 
*Inhibitors Ran today:
 
#50uM [2,3-bis(actyloxy)benzoic aicd] [PH003446]
 
#50uM [Luteolin] [AMB18511398]
 
#50uM [Morin] [AMB18511704]
 
#50uM [Robinetin] [AMB18511408]
 
#50uM [5-Hydroxy-2-methylbenzoic acid] [Aldrich 696366-1G]
 
  
 
==Data==
 
==Data==

Revision as of 14:40, 8 May 2013

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Data

417.png


410a.png

Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle