User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/03

From OpenWetWare
< User:Mary Mendoza‎ | Notebook‎ | CHEM572 Exp. Biological Chemistry II‎ | 2013‎ | 04
Revision as of 07:27, 5 April 2013 by Mary Mendoza (talk | contribs) (Preparation of Reagents)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Preparation of Reagents

  • 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
  • A new stock solution of 200 μM adenosine stock solution was also prepared.
  • For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
  • Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
Untitledx.png