Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/03"

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(Autocreate 2013/04/03 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II)
 
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==Entry title==
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==Preparation of Reagents==
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* 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
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* A new stock solution of 200 μM adenosine stock solution was also prepared.
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* For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
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* Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
  
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[[Image:Pe.png|center]]
  
 
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Revision as of 06:17, 5 April 2013

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Preparation of Reagents

  • 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
  • A new stock solution of 200 μM adenosine stock solution was also prepared.
  • For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
  • Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
Pe.png