User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

25 uM Adenosine Histogram Kinetic Assay Runs

  • The following procedures and data were taken from Dhea Patel.

Preparing 500uM Acetylsalicylic Acid Stock Solution

  • 13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:

Screen Shot 2013-03-26 at 12.39.06 PM.png

Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution

  • 4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
  • 2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
  • Screen Shot 2013-03-26 at 3.11.27 PM.png


  • Data Table of Volumes and Concentrations Used

File:20130326 Data Table.tiff


File:20130326 Data .tiff

  • The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.