Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26"

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(25 uM Adenosine Histogram Kinetic Assay Runs)
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==25 uM Adenosine Histogram Kinetic Assay Runs==
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* The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]].
 
  
 
==Preparing 500uM Acetylsalicylic Acid Stock Solution==
 
==Preparing 500uM Acetylsalicylic Acid Stock Solution==

Revision as of 14:26, 8 May 2013

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Preparing 500uM Acetylsalicylic Acid Stock Solution

  • 13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:

Screen Shot 2013-03-26 at 12.39.06 PM.png


Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution

  • 4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
  • 2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
  • Screen Shot 2013-03-26 at 3.11.27 PM.png

Kinetics

  • Data Table of Volumes and Concentrations Used

File:20130326 Data Table.tiff

Data

File:20130326 Data .tiff

  • The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.