Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26"

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==25 uM Adenosine Histogram Kinetic Assay Runs==
 
* The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]].
 
  
==Preparing 500uM Acetylsalicylic Acid Stock Solution==
 
*13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:
 
[[Image:Screen_Shot_2013-03-26_at_12.39.06_PM.png]]
 
  
  
==Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution==
 
*4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
 
*2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
 
*[[Image:Screen_Shot_2013-03-26_at_3.11.27_PM.png]]
 
  
==Kinetics==
 
*Data Table of Volumes and Concentrations Used
 
[[Image:20130326_Data_Table.tiff|px200]]
 
  
==Data==
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[[Image:20130326_Data_.tiff]]
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*The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.
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