Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26"

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(Autocreate 2013/03/26 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II)
 
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==Entry title==
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==25 uM Adenosine Histogram Kinetic Assay Runs==
* Insert content here...
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* The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]].
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==Preparing 500uM Acetylsalicylic Acid Stock Solution==
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*13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:
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[[Image:Screen_Shot_2013-03-26_at_12.39.06_PM.png]]
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==Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution==
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*4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
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*2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
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*[[Image:Screen_Shot_2013-03-26_at_3.11.27_PM.png]]
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==Kinetics==
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*Data Table of Volumes and Concentrations Used
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[[Image:20130326_Data_Table.tiff|px200]]
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==Data==
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[[Image:20130326_Data_.tiff]]
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*The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.  
  
  

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25 uM Adenosine Histogram Kinetic Assay Runs

  • The following procedures and data were taken from Dhea Patel.

Preparing 500uM Acetylsalicylic Acid Stock Solution

  • 13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:

Screen Shot 2013-03-26 at 12.39.06 PM.png


Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution

  • 4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
  • 2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
  • Screen Shot 2013-03-26 at 3.11.27 PM.png

Kinetics

  • Data Table of Volumes and Concentrations Used

File:20130326 Data Table.tiff

Data

File:20130326 Data .tiff

  • The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.