User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20

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  • It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials.
  • From Sigma, 500 μM Kaempferol (C15H10O6, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO).
  • A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system.
  • After the three trials of 40 μM adenosine, aspirin trials were conducted.
  • The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
  • Kaempferol trials were started.
  • After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM. In addition, cuvettes will also contain 300 μL of DMSO.
  • For the following week, a new aspiring stock solution will be prepared.

adenosine vs. aspirin

  • The histogram below displays the non-inhibitory effect of aspirin.



  • The data below was taken from Dhea Patel.
  • To measure the mass of Kaempferol and the volume of DMSO:

Screen Shot 2013-03-20 at 1.45.50 PM.png

  • Table of volumes and concentrations:

File:Kinetics Histogram table.tiff