Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20"

From OpenWetWare
Jump to: navigation, search
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
* It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials.
* From Sigma, 500 μM Kaempferol (C<sub>15</sub>H<sub>10</sub>O<sub>6</sub>, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO).
* A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system.
* After the three trials of 40 μM adenosine, aspirin trials were conducted.
* The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
* Kaempferol trials were started.
* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM. In addition, cuvettes will also contain 300 μL of DMSO.
* For the following week, a new aspiring stock solution will be prepared.
==adenosine vs. aspirin==
==adenosine vs. aspirin==

Revision as of 14:25, 8 May 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

adenosine vs. aspirin

  • The histogram below displays the non-inhibitory effect of aspirin.



  • The data below was taken from Dhea Patel.
  • To measure the mass of Kaempferol and the volume of DMSO:

Screen Shot 2013-03-20 at 1.45.50 PM.png

  • Table of volumes and concentrations:

File:Kinetics Histogram table.tiff