Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20"

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==Histogram==
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* It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials.
 
* From Sigma, 500 μM Kaempferol (C<sub>15</sub>H<sub>10</sub>O<sub>6</sub>, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO).
 
* A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system.
 
* After the three trials of 40 μM adenosine, aspirin trials were conducted.
 
* The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
 
* Kaempferol trials were started.
 
* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM. In addition, cuvettes will also contain 300 μL of DMSO.
 
* For the following week, a new aspiring stock solution will be prepared.
 
  
 
==adenosine vs. aspirin==
 
==adenosine vs. aspirin==

Revision as of 14:25, 8 May 2013

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adenosine vs. aspirin

  • The histogram below displays the non-inhibitory effect of aspirin.

Bar2.png

Tables

  • The data below was taken from Dhea Patel.
  • To measure the mass of Kaempferol and the volume of DMSO:

Screen Shot 2013-03-20 at 1.45.50 PM.png

  • Table of volumes and concentrations:

File:Kinetics Histogram table.tiff