Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/19"

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(75 μM Aspirin inhibitor)
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==75 μM Aspirin inhibitor==
 
* The following procedures were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/19|Dhea Patel]].
 
  
*Inhibitor kinetics using 75uM acetylsalicylic acid was performed.
 
*Adenosine stock solutions were prepared using the following table:
 
[[Image:Screen_Shot_2013-03-19_at_1.25.12_PM.png]]
 
  
*The volumes and concentrations added to each cuvette is outlined in the following table:
 
[[Image:Screen_Shot_2013-03-19_at_1.25.03_PM.png]]
 
  
==Data==
 
 
[[Image:75uM_inhibitor.tiff]]
 
 
The Km value was lower than anticipated.
 
 
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Revision as of 14:24, 8 May 2013

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