User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/27

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Objective

  • to begin ADA + Inhibitor kinetics

Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

Screen Shot 2013-02-27 at 12.43.29 PM.png

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.
  • ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:

Screen Shot 2013-02-27 at 2.57.04 PM.png

Data

File:20130227 ADA Kinetics 3T.tiff

File:20130227 Lineweaver Burk ADA Kinetics 3T.tiff

File:20130227 average ADA Kinetics 3T.tiff