Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/27"

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(ADA Kinetic Assay with the inhibitor acetylsalicylic acid)
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==ADA Kinetic Assay with the inhibitor acetylsalicylic acid==
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* The last concentration of adenosine (40 μM) for the zero point of trial 3 was run.
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*Taken from [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/02/27|Dhea Patel's Notebook]]. Everything was done together.
* The acetylsalicylic acid stock solution was prepared by dissolving 13.3 mg of the white, granular solid into 492.1551 mL of 0.05 M sodium phosphate buffer at pH 7.4.
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==Objective==
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*to begin ADA + Inhibitor kinetics
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==Description==
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*150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:
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[[Image:Screen_Shot_2013-02-27_at_12.43.29_PM.png]]
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*25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
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*More 0.05M phosphate buffer was prepared according to the procedure outlined in [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/01/23| January's entry]].
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*ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:
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[[Image:Screen_Shot_2013-02-27_at_2.57.04_PM.png]]
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==Data==
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[[Image:20130227_ADA_Kinetics_3T.tiff|px200]]
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[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff|px200]]
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[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]]
  
  

Revision as of 12:06, 27 February 2013

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Objective

  • to begin ADA + Inhibitor kinetics

Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

Screen Shot 2013-02-27 at 12.43.29 PM.png

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.
  • ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:

Screen Shot 2013-02-27 at 2.57.04 PM.png

Data

File:20130227 ADA Kinetics 3T.tiff

File:20130227 Lineweaver Burk ADA Kinetics 3T.tiff

File:20130227 average ADA Kinetics 3T.tiff