User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/27: Difference between revisions

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*Taken from [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/02/27|Dhea Patel's Notebook]]. Everything was done together.  
*Taken from [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/02/27|Dhea Patel's Notebook]]. Everything was done together.  


==Objective==
 
*to begin ADA + Inhibitor kinetics


==Description==
==Description==

Revision as of 15:21, 8 May 2013

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Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.
  • ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:

Data

px200

px200

px200



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