Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20"

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(Aspirin concentration for ADA Kinetic Assay)
(ADA Kinetic Assay for obtaining the zero point)
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==ADA Kinetic Assay for obtaining the zero point==
 
* The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
 
* UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
 
* The assays were prepared according to the data below.
 
  
[[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]]
 
 
* After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.
 
 
* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
 
* An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm.
 
* Thus, it was decided to run a full spectrum of adenosine at each given concentration specified above for accurate measurement of  the concentration of adenosine.
 
  
 
==Data==
 
==Data==

Revision as of 15:19, 8 May 2013

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Data

Concen1.png


AveVelo1.png


1adeno.png


Lin1.png


UV2.20.2.png