Difference between revisions of "User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20"

From OpenWetWare
Jump to: navigation, search
(ADA Kinetic Assay for obtaining the zero point)
(ADA Kinetic Assay for obtaining the zero point)
Line 17: Line 17:
 
* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
 
* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
 
* An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm.
 
* An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm.
 +
* Thus, it was decided to run a full spectrum of adenosine at each concentration specified above for accurate measurement of  the concentration.
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 12:22, 20 February 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

ADA Kinetic Assay for obtaining the zero point

  • The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
  • UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
  • The assays were prepared according to the data below.
Screen Shot 2013-02-19 at 2.46.05 PM.png
  • After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.
  • It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
  • An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10-4 of adenosine at 260 nm.
  • Thus, it was decided to run a full spectrum of adenosine at each concentration specified above for accurate measurement of the concentration.