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(ADA Kinetic Assay for obtaining the zero point)
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==ADA Kinetic Assay for obtaining the zero point==
 
* The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
 
* UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
 
* The assays were prepared according to the data below.
 
  
[[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]]
 
  
* After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.
 
  
* It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
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* An article was provided by Dr. Hartings with the molar extinction coefficient of adenosine at 261 nm.
 
  
 
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Latest revision as of 22:28, 26 September 2017

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