User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/19

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ADA Kinetic Assay with Acetylsalicylic Acid inhibitor

  • The objective for this laboratory period was to run ADA Kinetic assay with the addition of the inhibitor.
  • Acetylsalicylic acid (C9H8O4, MW 180.16) was obtained from Sigma-Aldrich, Co.
  • The assay of the acid is ≥ 99.0 %. The solid appeared as a white crystalline form having an absorbance of 230 nm.


  • The first trial conducted did not include the inhibitor for the purpose of obtaining a zero point.
  • There were observed nconsistencies among the 32, 39.7, 141.8, and 200 μM adenosine concentrations. Thus, new concentrations were calculated for adenosine.