User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13

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Revision as of 10:39, 13 February 2013 by Mary Mendoza (talk | contribs) (Trial 2 & 3 of ADA Kinetic Assay)
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Trial 2 & 3 of ADA Kinetic Assay

  • UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
  • Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
  • After baseline, an assay containing 150 μM adenosine was collected using the UV Probe.