User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/12: Difference between revisions
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*Temperature control: 25°C | *Temperature control: 25°C | ||
*Phosphate buffer in reference cell | *Phosphate buffer in reference cell | ||
*Ran kinetics on 250uM solution (20uL ADA, 600uL | *Ran kinetics on 250uM solution (20uL ADA, 600uL 1250 uM adenosine, and 2.38mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
**absorbance exceeded 4.00, so the assay was stopped and a new solution was prepared. | **absorbance exceeded 4.00, so the assay was stopped and a new solution was prepared. | ||
*Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12. | *Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8 uM adenosine, and 2.38mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
**dAbs was obtained and plotted vs. time in excel | **dAbs was obtained and plotted vs. time in excel | ||
*Ran kinetics on 6.4uM solution (20uL ADA, 600uL | *Ran kinetics on 6.4uM solution (20uL ADA, 600uL 32 uM adenosine, and 2.38mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
**dAbs was obtained and plotted vs. time in excel | **dAbs was obtained and plotted vs. time in excel | ||
*Ran kinetics on 16uM solution (20uL ADA, 600uL | *Ran kinetics on 16uM solution (20uL ADA, 600uL 80 uM adenosine, and 2.38mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
**dAbs was obtained and plotted vs. time in excel | **dAbs was obtained and plotted vs. time in excel | ||
*The following table describes the volumes and concentrations of the components added to the cuvette. | *The following table describes the volumes and concentrations of the components added to the cuvette. |
Revision as of 12:51, 13 February 2013
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ADA Kinetic Assay
Graphs
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