User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/05: Difference between revisions
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==ADA Kinetics Assay== | ==ADA Kinetics Assay== | ||
* The spectrophotometer used was the make of Shimadzu UV-2550 connected to a Shimadzu CPS-temperature controller. | * The objective of executing adenosine deaminase (ADA) kinetic assay is to obtain the slope or rate of the enzymatic reaction of the conversion of adenosine into inosine by ADA. | ||
*The spectrophotometer used was the make of Shimadzu UV-2550 connected to a Shimadzu CPS-temperature controller. | |||
* On the UV Probe system interface, the kinetics program was chosen under the Windows option. The parameters were adjusted from the method button. | * On the UV Probe system interface, the kinetics program was chosen under the Windows option. The parameters were adjusted from the method button. | ||
* The rest of the procedure is taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05|Dhea Patel's notebook]]. | * The rest of the procedure is taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05|Dhea Patel's notebook]]. |
Revision as of 08:51, 8 February 2013
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ADA Kinetics Assay
Calculations
Procedure
Data
Lineweaver-Burk Plot of the data obtained today.
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