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## Calculation of reagents for ADA kinetic assay

• It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

.030 $\displaystyle \frac{mol}{L}$ mol of adenosine × $\displaystyle \frac{267.24 g}{1 mol}$ = $\displaystyle \frac{8.0172 g}{L}$ × .010 L = 0.0802 g of adenosine

The calculations on the bottom are courtesy of Dhea Patel.

The calculation of for the buffer was formulated by Catherine Koenigsknecht

• Procedure: Buffer
1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)