Difference between revisions of "User:Manish Butte/Notebook/test notebook/2009/05/12"

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==Entry title==
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==P14 Cytotoxicity assay after PDL1 ligation==
 
* Purify P14 CD8 T cells, culture overnight in anti-cd3 (2 ug/ml) + 2h11 or ratIgG2b (40 ug/mL), replicating experiment from 11/08.
 
* Purify P14 CD8 T cells, culture overnight in anti-cd3 (2 ug/ml) + 2h11 or ratIgG2b (40 ug/mL), replicating experiment from 11/08.
 
1. coat 96 well flat with 2 ug/mL 2C11 (5.53 g/L stock) plus either 2h11 (1.26 g/L stock) or ratIgG2b (from alison 10+ mg/mL stock) at 40 ug/mL
 
1. coat 96 well flat with 2 ug/mL 2C11 (5.53 g/L stock) plus either 2h11 (1.26 g/L stock) or ratIgG2b (from alison 10+ mg/mL stock) at 40 ug/mL

Revision as of 02:48, 13 May 2009

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P14 Cytotoxicity assay after PDL1 ligation

  • Purify P14 CD8 T cells, culture overnight in anti-cd3 (2 ug/ml) + 2h11 or ratIgG2b (40 ug/mL), replicating experiment from 11/08.

1. coat 96 well flat with 2 ug/mL 2C11 (5.53 g/L stock) plus either 2h11 (1.26 g/L stock) or ratIgG2b (from alison 10+ mg/mL stock) at 40 ug/mL